To fix this, right click on a chromatogram, select properties, it should say opens with BioEdit, hit change, browse to the Chromas executable, select it, choose always open with this program, hit ok.īehavior of BioEdit ver. Sequence editing using BioEditĪdjust the size of the chromatogram trace with the Horizontal scale and Vertical ttutorial bars to the top left of the image. This creates a duplicate sequence that can be edited without changing the original sequence. I manually align them and tutoril for obvious missing bases and either correct them or add a gap to preserve the alignment. Select the N and replace it by typing in the appropriate base. Eventually the forwards will start to be a poor match to the reverses. I use this feature on nearly every dataset I create. Go back to your BioEdit file with all your sequences which should still have the original sequences highlightedpaste the sequences control-sthen delete the selected sequences control-dthus replacing the newly edited ones and removing the originals. At that point I finish my consensus futorial. I usually import all the forwards and reverses into a new BioEdit file. MEGA also has an alignment editor, but I’ve not really used it very much. BioEdit can also edit chromatograms, but I find Chromas to be nicer. BioEdit is a mouse-driven, easy-to-use sequence alignment editor and sequence analysis program designed and written by a graduate student. This is likely to be the final release of BioEdit. North Carolina State University, Department of Microbiology.
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